Proteomics

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SUMOylation regulates the metaphase-anaphase transition by stabilizing sister kinetochore biorientation

ABSTRACT: The accurate segregation of chromosomes during mitosis relies on the attachment of sister chromatids to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore-microtubule remains unclear. Here we identify SUMOylation as a mechanism that promotes anaphase onset upon biorientation. SUMO ligases modify the tension-sensing pericentromere-localized chromatin protein, shugoshin, to stabilize bioriented sister kinetochore-microtubule attachments and allow entry into anaphase. SUMOylation of shugoshin prevents binding to protein phosphatase 2A (PP2A), while enhancing chromosome passenger complex (CPC) interaction and removal from centromeres. Dissociation of the shugoshin-PP2A interaction is important for stabilizing sister kinetochore biorientation. Therefore, SUMOylation modulates protein interactions within pericentromeres to allow a signalling switch in response to biorientation.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Mike Tatham  

LAB HEAD: Ronald T. Hay

PROVIDER: PXD015565 | Pride | 2021-05-06

REPOSITORIES: Pride

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20150407_10_qEx_TT_T150_P4_Claudia_inGel_gluC_s1.raw Raw
20150407_13_qEx_TT_T150_P4_Claudia_inGel_gluC_s2.raw Raw
20150407_16_qEx_TT_T150_P4_Claudia_inGel_gluC_s3.raw Raw
20150407_19_qEx_TT_T150_P4_Claudia_inGel_gluC_s4.raw Raw
20150407_24_qEx_TT_T150_P4_Claudia_inGel_trp_s1.raw Raw
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Publications

During mitosis, sister chromatids attach to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension, which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore-microtubule attachments remains unclear. Here we show that SUMOylation dampens error correction to allow stable sister kinetochore biorientation and t  ...[more]