DirectMS1: MS/MS-free identification of 1000 proteins of cellular proteomes in 5 minutes
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Cell Culture, Hela Cell
SUBMITTER: 
Mark Ivanov
LAB HEAD: Mikhail Vladimirovich Gorshkov
PROVIDER: PXD015669 | Pride | 2020-02-27
REPOSITORIES: Pride
Publications
DirectMS1: MS/MS-Free Identification of 1000 Proteins of Cellular Proteomes in 5 Minutes.
Ivanov Mark V MV Bubis Julia A JA Gorshkov Vladimir V Tarasova Irina A IA Levitsky Lev I LI Lobas Anna A AA Solovyeva Elizaveta M EM Pridatchenko Marina L ML Kjeldsen Frank F Gorshkov Mikhail V MV
Analytical chemistry 20200305 6
Proteome characterization relies heavily on tandem mass spectrometry (MS/MS) and is thus associated with instrumentation complexity, lengthy analysis time, and limited duty cycle. It was always tempting to implement approaches that do not require MS/MS, yet they were constantly failing to achieve a meaningful depth of quantitative proteome coverage within short experimental times, which is particularly important for clinical or biomarker-discovery applications. Here, we report on the first succe ...[more]
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