The protein interactomes of AtNHR2A and AtNHR2B unraveled common and specialized functions in plant immunity integrating distinct biological processes

ABSTRACT: AtNHR2A (Arabidopsis thaliana nonhost resistance 2A) and AtNHR2B (Arabidopsis thaliana nonhost resistance 2B) are two proteins required in nonhost resistance, a broad spectrum mechanism of plant immunity that protects plants against pathogens. AtNHR2A and AtNHR2B are localized to the cytoplasm, chloroplasts and other subcellular compartments of unknown identity. The multiple localizations of AtNHR2A and AtNHR2B, suggest that they are highly dynamic and versatile proteins likely participating in multiple biological processes. In spite of their importance, their specific functions have not been elucidated. Thus, to aid in the functional characterization of these proteins and identify the biological processes in which these proteins participate, we used immunoprecipitation coupled with mass spectrometry (IP-MS), to identify the proteins interacting with AtNHR2A and AtNHR2B and generate their interactome network. Further analysis of the AtNHR2A- and AtNHR2B-interacting proteins using published empirical information revealed that AtNHR2A and AtNHR2B function is not limited to plant immunity, highlighting that these proteins are hubs integrating defense outputs with other biological processes.

INSTRUMENT(S): LTQ Orbitrap, Bruker Daltonics amaZon series

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Rohana Liyanage  

LAB HEAD: Clemencia M. Rojas

PROVIDER: PXD015894 | Pride | 2020-03-10

REPOSITORIES: Pride