Proteomics

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Plasma proteomic profiling of normal and over-conditioned dairy cows during the transition from late pregnancy to early lactation by LC-MS/MS


ABSTRACT: The objective of this study was to apply a quantitative proteomic approach to investigate changes in the plasma proteome of normal and over-conditioned dairy cows during the transition period. We used an animal model with a targeted difference in body condition score (BCS) at calving. For this, multiparous Holstein cows were allocated to two groups 15 weeks before anticipated calving and were fed differently to reach either high (HBCS: 7.2 NEL MJ/kg dry matter (DM) or normal BCS (NBCS: 6.8 NEL MJ/kg DM) until dry-off. Thereafter, both groups were fed identical diets. The targeted BCS difference was sustained during the dry period (7 weeks before calving) and the subsequent 12 weeks of lactation. Plasma samples (n = 5) from d -49, +7, and +21 relative to parturition were used from both groups (each n = 5) for proteome profiling in aby applying quantitative Tandem Mass Tags (TMT) approach.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Plasma

DISEASE(S): Increased Body Weight

SUBMITTER: Anita Horvatic  

LAB HEAD: Peter David Eckersall

PROVIDER: PXD015958 | Pride | 2020-04-16

REPOSITORIES: Pride

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Publications

Plasma proteomic profiling and pathway analysis of normal and overconditioned dairy cows during the transition from late pregnancy to early lactation.

Ghaffari Morteza H MH   Schuh Katharina K   Kuleš Josipa J   Guillemin Nicolas N   Horvatić Anita A   Mrljak Vladimir V   Eckersall Peter David PD   Dusel Georg G   Koch Christian C   Sadri Hassan H   Sauerwein Helga H  

Journal of dairy science 20200312 5


This study applied a quantitative proteomics approach along with bioinformatics analyses to investigate changes in the plasma proteome of normal and overconditioned dairy cows during the transition period. Fifteen weeks before their anticipated calving date, 38 multiparous Holstein cows were selected based on their current and previous body condition scores (BCS) and allocated to either a high or a normal BCS group (19 cows each). They received different diets until dry-off to reach targeted dif  ...[more]

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