Dataset Information

Host-defence peptides caerin 1.1 and 1.9 stimulate TNF-alpha-dependent apoptosis in human cervical cancer HeLa cells

ABSTRACT: TNFα pathway has been recognised as one of the most important signalling pathways characterising molecules with anti-cancer activity, due to it closely relates to cell survival as well as apoptosis. Isolated from the glandular secretion of Australian tree frogs, the genus Litoria, host-defence caerin peptides have been previously shown to have multiple biological activities, including anti-proliferation of several cancer cell lines, though the mechanism remains unclear. In this study, we used multiple immunoassays, confocal microscopy in conjunction with TMT-labelling quantitative proteomics to investigate the anti-proliferative activities of caerin 1.1 and 1.9 against cervical cancer HeLa cells. The proteins of cells and cell growth environment post the treatments were comparatively analysed, respectively. We found that both caerin 1.1 and 1.9 highly inhibited HeLa cell proliferation with a significant additive effect. The PPI analysis confirmed that the interactions among the significantly regulated proteins were highly intensive. Besides translation, folding and localisation of proteins and RNA processing, apoptosis process was significantly enriched post the treatments. The apoptosis process was resulted by TNFα signalling, confirmed by the elevated levels of CASP3 and CASP9. Moreover, EGFR1 and androgen receptor pathways appeared inhibited. The activation of TCR pathway derived from the quantitation results further implies the likelihood of recruiting more T cells to the cell growth environment post the treatment and more sensitive to T cell mediated killing of Hela cells.

INSTRUMENT(S): X500R QTOF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Hela Cell

DISEASE(S): Breast Cancer

SUBMITTER: Tianfang Wang

LAB HEAD: Tianfang Wang

PROVIDER: PXD015975 | Pride | 2020-08-27

REPOSITORIES: Pride

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Publications

Host-Defense Peptides Caerin 1.1 and 1.9 Stimulate TNF-Alpha-Dependent Apoptotic Signals in Human Cervical Cancer HeLa Cells.

Ni Guoying G Chen Shu S Chen Mo M Wu Jialing J Yang Binbin B Yuan Jianwei J Walton Shelley F SF Li Hejie H Wei Ming Q MQ Wang Yuejian Y Chen Guoqiang G Liu Xiaosong X Wang Tianfang T

Frontiers in cell and developmental biology 20200731

Host defense caerin 1.1 and 1.9 peptides, isolated from the glandular secretion of Australian tree frogs, the genus Litoria, have been previously shown to have multiple biological activities, including the inhibition of human papillomavirus (HPV) 16 early protein E7 transformed murine as well as human cancerous cell proliferation both in vitro and in vivo. However, the mechanism underlying their anti-proliferative activities against HPV18+ cervical cancer HeLa cells remains ...[more]

PMID: 32850805

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Project description:Schistosomiasis, caused by infection with Schistosoma trematode parasites, is one of the deadliest neglected tropical diseases in the world. The Schistosoma lifecycle involves the miracidia infection of an intermediate molluscan host, such as Biomphalaria glabrata. Dispersing B. glabrata attractant chemicals has been considered a method of minimising host infections. The attractiveness of B. glabrata is known to be reduced following infection; however, it remained unclear how the duration of infection affects attractiveness. Identifying excretory-secretory proteins (ESPs) abundant in attractive snail conditioned water (SCW) may facilitate the identification of attractants. This study obtained SCW ESPs from naïve snails and at different time-points post-miracidia exposure (PME; 16h-, 1 week, 2 weeks and 3 weeks), to identify those ESPs mediating Schistosome mansoni miracidia behavioural changes. The ESPs were quantitatively identified from all SCW samples using a label-free proteomic method and genome-derived protein databases of B. glabrata and S. mansoni. We found that changes in miracidia behaviour were similar upon exposure to naïve and 3W-PME SCW, including increased circling and quantity of miracidia in the field of view. Of the ESPs identified, 21 were shared in SCW of naïve and 3W-PME, and considered attractant candidates, including acetylcholine-binding protein and immune-related proteins such as biomphalysins and thioester-containing protein 1. Some biological processes were exclusive to attractant candidates, including obsolete pathogenesis and transmembrane transport activity. This suggests that compromised production of these proteins may diminish host attractiveness to miracidia. Additionally, several immune proteins were identified exclusively at 16-PME and 1W-PME, providing further information into changing B. glabrata immune responses throughout infection. These findings provide a list of attractant candidates to potentially decrease B. glabrata infection and minimise schistosomiasis.

Dataset Information

Host-defence peptides caerin 1.1 and 1.9 stimulate TNF-alpha-dependent apoptosis in human cervical cancer HeLa cells

Publications

Host-Defense Peptides Caerin 1.1 and 1.9 Stimulate TNF-Alpha-Dependent Apoptotic Signals in Human Cervical Cancer HeLa Cells.

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