Comparative Differential Cuproproteomes of Rhodobacter capsulatus

ABSTRACT: In this study, we achieved a global view of Cu-responsive changes in the prokaryotic model organism Rhodobacter capsulatus using label-free quantitative differential proteomics. Semi-aerobically grown cells under heterotrophic conditions in minimal medium (~ 0.3 M Cu, optimal for growth) were compared with cells grown similarly but supplemented with either 5 M Cu or with 5 mM of the Cu-chelator bathocuproine sulfonate. Mass spectrometry based bottom-up proteome analyses of unfractionated cell lysates identified with high confidence 2430 of the 3632 putative proteins encoded by the genome.

INSTRUMENT(S):

ORGANISM(S): Rhodobacter Capsulatus (strain Atcc Baa-309 / Nbrc 16581 / Sb1003)

TISSUE(S): Photosynthetic Cell

SUBMITTER: Nur Selamoglu  

LAB HEAD: Fevzi Daldal

PROVIDER: PXD016079 | Pride | 2020-06-28

REPOSITORIES: Pride