Proteomics

Dataset Information

0

Staphylococcus aureus surface LC-MS/MS analysis


ABSTRACT: Staphylococcus aureus causes treatment-resistant infections, to which biofilm formation and bacterial persistence contribute greatly. This study aims at the large-scale characterization of mechanisms behind its prolonged persistence in hostile environments and capability to evade host defenses. We carried out the analysis of surface-exposed proteins in S. aureus biofilms prepared from two types of inocula; metabolically active, levofloxacin-susceptible culture and stationary phase, levofloxacin-tolerant culture. Age-synchronized planktonic control cultures provided a reference to facilitate the identification of growth mode-specific surface-expression patterns.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus aureus  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Tuula Nyman  

LAB HEAD: Tuula Nyman

PROVIDER: PXD016344 | Pride | 2020-01-20

REPOSITORIES: pride

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Publications

Growth Mode and Physiological State of Cells Prior to Biofilm Formation Affect Immune Evasion and Persistence of Staphylococcus aureus.

Savijoki Kirsi K   Miettinen Ilkka I   Nyman Tuula A TA   Kortesoja Maarit M   Hanski Leena L   Varmanen Pekka P   Fallarero Adyary A  

Microorganisms 20200112 1


The present study investigated Staphylococcus aureus ATCC25923 surfaceomes (cell surface proteins) during prolonged growth by subjecting planktonic and biofilm cultures (initiated from exponential or stationary cells) to label-free quantitative surfaceomics and phenotypic confirmations. The abundance of adhesion, autolytic, hemolytic, and lipolytic proteins decreased over time in both growth modes, while an opposite trend was detected for many tricarboxylic acid (TCA) cycle, reactive oxygen spec  ...[more]

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