GdpS contributes to Staphylococcus aureus biofilm formation by regulation of eDNA release

ABSTRACT: In Staphylococcus aureus, the role of the GGDEF domain containing protein GdpS remains poorly understood. Previous studies reported that gdpS mutant strains had decreased biofilm formation due to changes in icaADBC expression that were independent of cyclic-di-GMP levels. We deleted gdpS in three unrelated S. aureus isolates, and analyzed the resultant mutants for alterations in biofilm formation, metabolism and transcription. Dynamic imaging during biofilm development showed that GdpS inhibited early biofilm formation in only two out of the three strains examined, without affecting bacterial survival. However, quantification of biofilm formation using crystal violet staining revealed that inactivation of gdpS affected biofilm formation in all three studied strains. Extraction of metabolit

ORGANISM(S): Staphylococcus aureus

SUBMITTER: FRANCOIS Patrice 

PROVIDER: E-GEOD-48656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress