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Alteration of the plasma peptidome of gastrectomised patients during an OGTT

ABSTRACT: Performing gastric bypass surgery on patients severely changes their gut hormone response to glucose. The plasma concentrations of Insulin and a number of associated incretin peptides are significantly raised shortly after ingestion of glucose. Typically the plasma concentrations of these peptides are monitored using immunoassays. A simple and high throughput plasma peptidomics approach was used to simulatneously monitor a large number of these peptides. Comparison of gastresctomised and control individuals showed significant increases of insulin, glucagon gene products, PYY and neurotensin.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Postgastrectomy Syndrome

SUBMITTER: Richard Kay

LAB HEAD: Fiona Gribble, Frank Reimann

PROVIDER: PXD016690 | Pride | 2020-06-05

REPOSITORIES: Pride

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Mass spectrometric characterisation of the circulating peptidome following oral glucose ingestion in control and gastrectomised patients.

Kay Richard G RG Foreman Rachel E RE Roberts Geoff P GP Hardwick Richard R Reimann Frank F Gribble Fiona M FM

Rapid communications in mass spectrometry : RCM 20200901 18

<h4>Rationale</h4>Meal ingestion triggers secretion of a variety of gut and endocrine peptides important in diabetes research which are routinely measured by immunoassays. However, similarities between some peptides (glucagon, oxyntomodulin and glicentin) can cause specificity issues with immunoassays. We used a liquid chromatography/tandem mass spectrometry (LC/MS/MS) methodology to unambiguously monitor multiple gut peptides in human plasma.<h4>Methods</h4>A simple acetonitrile-based protein p ...[more]

PMID: 32492232

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