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UAG suppression in HEK293T cells

ABSTRACT: HEK293T cells, stably transfected with pyrrolysine UAG suppressor tRNA and aminoacyl tRNA synthetase, for the co-translational incorporation of Boc-protected lysine.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Eyal Arbely

LAB HEAD: Eyal Arbely

PROVIDER: PXD016728 | Pride | 2020-02-24

REPOSITORIES: Pride

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			Action	DRS
	HF1_9641_EAR_boc-nt2.raw	Raw
	HF1_9641_EAR_boc-nt3_190619204817.raw	Raw
	HF1_9641_EAR_boc-nt4.raw	Raw
	HF1_9641_EAR_boc-nt5_190620025225.raw	Raw
	HF1_9641_EAR_boc1.raw	Raw

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1 - 5 of 9

Publications

Translational read-through promotes aggregation and shapes stop codon identity.

Kramarski Lior L Arbely Eyal E

Nucleic acids research 20200401 7

Faithful translation of genetic information depends on the ability of the translational machinery to decode stop codons as termination signals. Although termination of protein synthesis is highly efficient, errors in decoding of stop codons may lead to the synthesis of C-terminally extended proteins. It was found that in eukaryotes such elongated proteins do not accumulate in cells. However, the mechanism for sequestration of C-terminally extended proteins is still unknown. Here we show that 3'- ...[more]

PMID: 32128584

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