Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)
TISSUE(S): Whole Plant
SUBMITTER: Chuan-Chih Hsu
LAB HEAD: Zhi-Yong Wang
PROVIDER: PXD017085 | Pride | 2023-05-10
REPOSITORIES: Pride
Kim Tae-Wuk TW Park Chan Ho CH Hsu Chuan-Chih CC Kim Yeong-Woo YW Ko Yeong-Woo YW Zhang Zhenzhen Z Zhu Jia-Ying JY Hsiao Yu-Chun YC Branon Tess T Kaasik Krista K Saldivar Evan E Li Kevin K Pasha Asher A Provart Nicholas J NJ Burlingame Alma L AL Xu Shou-Ling SL Ting Alice Y AY Wang Zhi-Yong ZY
The Plant cell 20230301 3
Elucidating enzyme-substrate relationships in posttranslational modification (PTM) networks is crucial for understanding signal transduction pathways but is technically difficult because enzyme-substrate interactions tend to be transient. Here, we demonstrate that TurboID-based proximity labeling (TbPL) effectively and specifically captures the substrates of kinases and phosphatases. TbPL-mass spectrometry (TbPL-MS) identified over 400 proximal proteins of Arabidopsis thaliana BRASSINOSTEROID-IN ...[more]