Functionalized Peptidisc Scaffolds Enable Affinity Purification of the Membrane Proteome

ABSTRACT: We reconstituted the total E. coli membrane proteome into His-tagged peptidiscs. We then performed an affinity purification to enrich the bona fide membrane proteome away from soluble contaminants which co-sediment with cellular membranes after cell lysis. As a case study, we employ this method to survey changes to the membrane proteome caused by altered gene expression.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: David Rattray  

LAB HEAD: Franck Van Hoa Duong

PROVIDER: PXD017242 | Pride | 2022-04-11

REPOSITORIES: Pride