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Protein EV contents from C. elegans

ABSTRACT: The hermaphrodite strain N2 (Bristol), fed with Escherichia coli OP50, was maintained and synchronized. Young adults were thoroughly washed before incubations for 5 h or 24 h in M9 buffer. Between 35,500 and 158,000 worms were used for each experiment. After incuabtion, supernatants were processed by differential centrigugation/ultracentrifugation to pellet extracellular vesicles and characterization of the protein contents by mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli Caenorhabditis Elegans

TISSUE(S): Cell Culture

SUBMITTER: Lucienne Tritten

LAB HEAD: Lucienne Tritten

PROVIDER: PXD017352 | Pride | 2020-05-04

REPOSITORIES: Pride

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Extracellular Vesicle-Contained microRNA of <i>C. elegans</i> as a Tool to Decipher the Molecular Basis of Nematode Parasitism.

Duguet Thomas B TB Soichot Julien J Kuzyakiv Rostyslav R Malmström Lars L Tritten Lucienne L

Frontiers in cellular and infection microbiology 20200525

Among the fundamental biological processes affected by microRNAs, small regulators of gene expression, a potential role in host-parasite communication is intriguing. We compared the miRNA complement of extracellular vesicles released by the free-living nematode <i>Caenorhabditis elegans</i> in culture to that of other adult parasitic nematodes. Expecting convergent functional roles for secreted miRNAs due to the common parasitic lifestyle of the organisms under investigation, we performed a miRN ...[more]

PMID: 32523895

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