Protein EV contents from C. elegans

ABSTRACT: The hermaphrodite strain N2 (Bristol), fed with Escherichia coli OP50, was maintained and synchronized. Young adults were thoroughly washed before incubations for 5 h or 24 h in M9 buffer. Between 35,500 and 158,000 worms were used for each experiment. After incuabtion, supernatants were processed by differential centrigugation/ultracentrifugation to pellet extracellular vesicles and characterization of the protein contents by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli Caenorhabditis Elegans

TISSUE(S): Cell Culture

SUBMITTER: Lucienne Tritten  

LAB HEAD: Lucienne Tritten

PROVIDER: PXD017352 | Pride | 2020-05-04

REPOSITORIES: Pride