Proteomic profiling of RNA binding proteins in mouse spermatogonial stem cell
Ontology highlight
ABSTRACT:
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Stem Cell
SUBMITTER: 
Tao Zhou
LAB HEAD: Xin Wu
PROVIDER: PXD017377 | Pride | 2020-03-19
REPOSITORIES: Pride
Publications
mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation.
Du Guihua G Wang Xinrui X Luo Mengcheng M Xu Weiya W Zhou Tao T Wang Mei M Yu Luping L Li Lufan L Cai Li'e L Wang P Jeremy PJ Zhong Li John J Oatley Jon M JM Wu Xin X
Development (Cambridge, England) 20200412 8
Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA-binding proteins (RBPs) in regulation of the biological activities of this population. Proteins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking and profiled by proteomics (termed mRBPome capture), ...[more]
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