Proteomics

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Label-free based Proteomic analysis of secreted proteins from Mycoplasma bovis HB0801 and its attenuated strain P150

ABSTRACT: This study was aimed to perform a comparative analysis on potential secretory proteins between high (HB0801) and low (P150) virulent M.bovis strains. We employed the liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based label-free quantitative proteomic technology to identify and characterize the differential secretome profiles for two M.bovis strains. A total of 477 secretory proteins were identified in both two strains and 165 deferentially expressed in HB0801 over 1.5-folds higher than P150.

INSTRUMENT(S):

ORGANISM(S): Mycoplasma Bovis Hb0801

SUBMITTER: hui zhang  

LAB HEAD: Aizhen Guo

PROVIDER: PXD017700 | Pride | 2021-09-08

REPOSITORIES: Pride

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P17188_MycoplasmabovisHB0801-A1.raw Raw
P17188_MycoplasmabovisHB0801-A2.raw Raw
P17188_MycoplasmabovisHB0801-A3.raw Raw
P17188_MycoplasmabovisP150-B1.raw Raw
P17188_MycoplasmabovisP150-B2.raw Raw
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Publications

Comparative Secretome Analyses of <i>Mycoplasma bovis</i> Virulent and Attenuated Strains Revealed MbovP0145 as a Promising Diagnostic Biomarker.

Zhang Hui H   Hu Guyue G   Lu Doukun D   Zhao Gang G   Zhang Yiqiu Y   Zubair Muhammad M   Chen Yingyu Y   Hu Changmin C   Chen Xi X   Chen Jianguo J   Chen Huanchun H   Yang Liguo L   Guo Aizhen A  

Frontiers in veterinary science 20210618

Mycoplasmas are successful pathogens both in humans as well as in animals. In cattle, <i>Mycoplasma bovis</i> (<i>M. bovis</i>) is known to be responsible for serious health complications, including pneumonia, mastitis, and arthritis. However, <i>M. bovis</i> pathogenesis remains unclear. Secreted proteins of <i>M. bovis</i> could influence infection and modify host defense signaling pathways after they enter their extracellular space in the host micro-environment. Therefore, this study was aime  ...[more]

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