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Maturation kinetics of a multiprotein complex revealed by metabolic labeling

ABSTRACT: The project aims at computing in vivo co-assembly kinetics from metabolomic-inspired approaches. In short, we performed reciprocal pull-down experiments from complex cellular components at different time points following pulsed-SILAC metabolic labeling. We applied the method to 320 pairs of yeast nucleoporin (NUP) proteins constituting the ~ 50 MDa Nuclear Pore Complex (NPC). Our analysis reveals a hierarchical principle of the NPC biogenesis where individual subcomplexes form on a minute time-scale, followed by their co-assembly from central to peripheral subunits in a ~ 1 hour long maturation process.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Ludovic Gillet

LAB HEAD: Evgeny Onischenko

PROVIDER: PXD018034 | Pride | 2020-10-16

REPOSITORIES: Pride

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			Action	DRS
	180912_LG_Evgeny81_1.raw	Raw
	180912_LG_Evgeny81_10b.raw	Raw
	180912_LG_Evgeny81_11.raw	Raw
	180912_LG_Evgeny81_12.raw	Raw
	180912_LG_Evgeny81_13.raw	Raw

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