Optogenetic Src Temporal Signaling
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF, Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Jason Conage-Pough
LAB HEAD: Forest Michael White
PROVIDER: PXD018162 | Pride | 2020-10-20
REPOSITORIES: Pride
Publications
Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity.
Shaaya Mark M Fauser Jordan J Zhurikhina Anastasia A Conage-Pough Jason E JE Huyot Vincent V Brennan Martin M Flower Cameron T CT Matsche Jacob J Khan Shahzeb S Natarajan Viswanathan V Rehman Jalees J Kota Pradeep P White Forest M FM Tsygankov Denis D Karginov Andrei V AV
eLife 20200923
Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical limitations. Here, we report an optogenetic approach that utilizes an engineered Light-Regulated (LightR) allosteric switch module to achieve tight spatiotemporal control of enzymatic activity. Using the tyrosine kinase Src as a model, we demonstrat ...[more]
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