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Identification of protein interaction partners of the lncRNA Lassie

ABSTRACT: The shear stress-regulated lncRNA LASSIE interferes with endothelial cell function as knockdown of Lassie affects apoptosis, proliferation and angiogenic sprouting in vitro. RNA-antisense purification and subsequent mass spectrometry analysis identifed junctional proteins (such as PECAM-1) as interactors of LASSIE . Additional functional analyses demonstrate a role of LASSIE in shear stress sensing and barrier function in endothelial cells, by stabilizing endothelial cell junctions through connection to the cytoskeleton.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig

LAB HEAD: Reinier Boon

PROVIDER: PXD018724 | Pride | 2021-07-22

REPOSITORIES: Pride

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Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function.

Stanicek Laura L Lozano-Vidal Noelia N Bink Diewertje Ilse DI Hooglugt Aukie A Yao Wenjie W Wittig Ilka I van Rijssel Jos J van Buul Jaap Diederik JD van Bergen Anke A Klems Alina A Ramms Anne Sophie AS Le Noble Ferdinand F Hofmann Patrick P Szulcek Robert R Wang ShengPeng S Offermanns Stefan S Ercanoglu Meryem Seda MS Kwon Hyouk-Bum HB Stainier Didier D Huveneers Stephan S Kurian Leo L Dimmeler Stefanie S Boon Reinier Abraham RA

Communications biology 20200526 1

Blood vessels are constantly exposed to shear stress, a biomechanical force generated by blood flow. Normal shear stress sensing and barrier function are crucial for vascular homeostasis and are controlled by adherens junctions (AJs). Here we show that AJs are stabilized by the shear stress-induced long non-coding RNA LASSIE (linc00520). Silencing of LASSIE in endothelial cells impairs cell survival, cell-cell contacts and cell alignment in the direction of flow. LASSIE associates with junction ...[more]

PMID: 32457386

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