Proteomics

Dataset Information

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Ubiquitin-mediated modulation of necroptotic effector MLKL

ABSTRACT: We aimed to identify lysine (K) residues that are endogenously ubiquitylated upon TNF-induced necroptosis. To this end, we used a quantitative mass spectrometry-based approach. Briefly, MDFs were treated with TSZ or left untreated and protein lysates were digested with trypsin into peptides. Ubiquitylated peptides bearing the di-glycine (K-GG) remnant were enriched by immunoprecipitation with anti-K-ε-GG antibody. A total of three biological replicates were prepared and the 6 samples were labelled with Tandem Mass Tags (TMT). Samples were mixed and analysing by Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS). Quantification was performed to determine the relative abundance of site-specific ubiquitylation events in each condition.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: James Wright  

LAB HEAD: Jyoti Choudhary

PROVIDER: PXD018857 | Pride | 2021-04-13

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
LRG02TMT6plex-V5.msf Msf
LRG02TMT6plex-V5.pdResult Other
LRG02TMT6plex-V5_PeptideGroups.txt Txt
LRG02TMT6plex-V5_Proteins.txt Txt
Lumos50cm_LRG02TMT6plex-P01.raw Raw
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Publications

Large-needle biopsy versus thin-needle biopsy in diagnostic pathology of liver diseases.

Röcken C C   Meier H H   Klauck S S   Wolff S S   Malfertheiner P P   Roessner A A  

Liver 20011201 6

<h4>Background/aims</h4>A study was carried out to determine whether thin-needle biopsy (TNB) yields enough material to study diffuse liver diseases.<h4>Methods</h4>Using TNB (20G and 21G) and a conventional Menghini-type large-needle biopsy (LNB; 17G), the amounts of tissue obtained and the histopathological diagnoses were compared. The biopsies were obtained by surgeons with a several-stroke method (17GS) and by physicians with a single-strike method (17GP, 20GP, 21GP). A total of 343 biopsy s  ...[more]

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