Dataset Information

Quantitative proteome, phosphoproteome and acetylome analysis of Arabidopsis thaliana root responses to persistent osmotic and salinity stress.

ABSTRACT: Environmental conditions contributing to abiotic stresses such as drought and salinity result in large annual economic losses around the world. As sessile organisms, plants cannot escape the environmental stresses they encounter, but instead must adapt to survive. Previous studies investigating osmotic and/or salt responses have largely focused on understanding short-term responses (0-1h) at the transcriptomic, proteomic and phosphoproteomic level; however, our understanding of intermediate to longer-term adaptation (24h - days) is relatively limited. In addition to protein abundance and phosphorylation changes, recent evidence suggests reversible protein acetylation may be important for abiotic stress responses. Therefore, to characterize the effects of osmotic and salt stress, we undertook a label-free proteomic and PTMomic analysis of Arabidopsis roots exposed to 300mM Mannitol and 150mM NaCl for 24 h. We quantitatively assessed protein abundance, phosphorylation and acetylation.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Richard Uhrig

LAB HEAD: Richard Uhrig

PROVIDER: PXD019139 | Pride | 2022-05-04

REPOSITORIES: Pride

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Publications

Quantitative Proteome and PTMome Analysis of Arabidopsis thaliana Root Responses to Persistent Osmotic and Salinity Stress.

Rodriguez Maria C MC Mehta Devang D Tan Maryalle M Uhrig Richard G RG

Plant & cell physiology 20211001 6

Abiotic stresses such as drought result in large annual economic losses around the world. As sessile organisms, plants cannot escape the environmental stresses they encounter but instead must adapt to survive. Studies investigating plant responses to osmotic and/or salt stress have largely focused on short-term systemic responses, leaving our understanding of intermediate to longer-term adaptation (24 h to d) lacking. In addition to protein abundance and phosphorylation changes, evidence suggest ...[more]

PMID: 34059891

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Project description:Pathak2013 - MAPK activation in response to various abiotic stresses MAPK activation mechanism in response to various abiotic stress conditions, such as cold, salt, drought, H2O2, heavy metal and ethylene, in plants This model is described in the article: Modeling of the MAPK machinery activation in response to various abiotic and biotic stresses in plants by a system biology approach. Pathak RK, Taj G, Pandey D, Arora S, Kumar A. Bioinformation 2013; 9(9): 443-449 Abstract: Mitogen-Activated Protein Kinases (MAPKs) cascade plays an important role in regulating plant growth and development, generating cellular responses to the extracellular stimuli. MAPKs cascade mainly consist of three sub-families i.e. mitogen-activated protein kinase kinase kinase (MAPKKK), mitogen-activated protein kinase kinase (MAPKK) and mitogen activated protein kinase (MAPK), several cascades of which are activated by various abiotic and biotic stresses. In this work we have modeled the holistic molecular mechanisms essential to MAPKs activation in response to several abiotic and biotic stresses through a system biology approach and performed its simulation studies. As extent of abiotic and biotic stresses goes on increasing, the process of cell division, cell growth and cell differentiation slow down in time dependent manner. The models developed depict the combinatorial and multicomponent signaling triggered in response to several abiotic and biotic factors. These models can be used to predict behavior of cells in event of various stresses depending on their time and exposure through activation of complex signaling cascades. This model is hosted on BioModels Database and identified by: BIOMD0000000491 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Project description:In natural habitats, plants are often exposed to multiple stresses. Most studies, however, for plant abiotic stress responses analyzed those to individual stress but not combined stresses. In this report, we performed comparison analyses of gene expression to individual stresses, salt, osmotic and heat, and to a combination of these three stresses, which mimics arid conditions. We show here that the combined stress treatment induces unique gene expression pattern but not a simple reflection of the additive effects of individual stresses. First, the number of genes induced by combined stresses (150 mM NaCl, 200 mM mannitol and 35Â°C heat) was much smaller when compared to the sum of those induced by individual stress treatments, while the number of genes downregulated by multiple stresses was larger. A large number of genes induced by mannitol were not induced by multiple stresses, while those induced by salts were less affected in combined stress treatments. In addition, 125 genes, including13 for transcription factors, were found to be induced specifically by combined stress treatments. We report here that the plant response to a multi-stress environment represents an output of complex interactions between different stress aspects and signaling events of the various components of this environmental situation, and the determinative factor in this response is to avoid/minimize the antagonistic effects and to magnify the synergistic features of the imposed environmental challenges. Based on our results, we propose that genes that are highly induced by multiple treatments may be candidate for engineering stress tolerant crop plants. Wild-type plants of Arabidopsis thaliana were treated with three abiotic stresses, high salinity (150mM and 300mM), high osmotic pressure (200mM and 400mM mannitol) and heat (35Â°C), individually or simultaneously. Plants were treated with salt or mannitol for 16 hrs (first 1 hr and last 7 hrs are in light and other time in dark) or with heat for 4 hrs (in light) before sampling. There are three biologcal replicates.

Dataset Information

Quantitative proteome, phosphoproteome and acetylome analysis of Arabidopsis thaliana root responses to persistent osmotic and salinity stress.

Publications

Quantitative Proteome and PTMome Analysis of Arabidopsis thaliana Root Responses to Persistent Osmotic and Salinity Stress.

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