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Quantitative proteomic analysis of mouse cerebellar granule cells after knockdown of YTHDF1

ABSTRACT: To identify the target mRNAs of YTHDF1 in granule cells, we performed quantitative proteomic analysis. Mouse cerebellar granule cells were cultured and infected with lentiviral shYthdf1 or shCtrl. Proteins were extracted. After further processing, TMT-labeled peptides were subjected to LC-MS/MS analysis. We discovered 352 proteins that were differentially expressed altogether (change fold >1.2), among which 142 proteins were downregulated after YTHDF1 KD. This study provides a protein list which shows differentially expressed proteins after knockdown of YTHDF1 in granule cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebellum

SUBMITTER: Jun Yu

LAB HEAD: ShengJian Ji

PROVIDER: PXD019249 | Pride | 2022-02-15

REPOSITORIES: Pride

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The m6 A Readers YTHDF1 and YTHDF2 Synergistically Control Cerebellar Parallel Fiber Growth by Regulating Local Translation of the Key Wnt5a Signaling Components in Axons.

Yu Jun J She Yuanchu Y Yang Lixin L Zhuang Mengru M Han Peng P Liu Jianhui J Lin Xiaoyan X Wang Nijia N Chen Mengxian M Jiang Chunxuan C Zhang Yujia Y Yuan Yujing Y Ji Sheng-Jian SJ

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20211012 22

Messenger RNA m6 A modification is shown to regulate local translation in axons. However, how the m6 A codes in axonal mRNAs are read and decoded by the m6 A reader proteins is still unknown. Here, it is found that the m6 A readers YTHDF1 and YTHDF2 are both expressed in cerebellar granule cells (GCs) and their axons. Knockdown (KD) of YTHDF1 or YTHDF2 significantly increases GC axon growth rates in vitro. By integrating anti-YTHDF1&2 RIP-Seq with the ...[more]

PMID: 34643063

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