Dataset Information

A multidimensional proteomic strategy for the characterization of the human amnion proteome

ABSTRACT: The loss of fetal membrane (FM) integrity and function in early pregnancy can have devastating consequences for the fetus and the newborn. However, the current fragmentary knowledge of FM biology has largely hampered the development of preventive FM sealing and healing strategies. Here, by an optimized protein sample preparation and offline fractionation before LC-MS/MS analysis we present an exhaustive human term amnion proteome characterization. The more than 5000 identified proteins greatly outnumbered previous reports. A Gene-Set Enrichment Analysis (GSEA) depicted ‘extracellular matrix’ and ‘cell-substrate junction‘ as two significantly enriched categories. Therefore, protein-protein interaction plots using these categories enabled the establishment of novel potential amniotic membrane cell-to-ECM interactions. Together, this thorough human amnion proteome, additionally to providing a basis for the study of compromised and preterm ruptured fetal membranes, could be a stepping-stone for the development of novel healing-inducing biomaterials.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fetal Membrane, Amnion

SUBMITTER: Eva Avilla-Royo

LAB HEAD: PD Dr. sc. nat. Martin Ehrbar

PROVIDER: PXD019410 | Pride | 2021-11-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20190506_003_S196771_20190506_amnionUrea_D1C_trial2_Urea_extraction (F282664).mzid_20190506_003_S196771_20190506_amnionUrea_D1C_trial2_Urea_extraction_(F282664).pride.mgf.gz	Mzid
	20190506_003_S196771_20190506_amnionUrea_D1C_trial2_Urea_extraction (F282664).pride.mztab.gz	Mztab
	20190506_003_S196771_20190506_amnionUrea_D1C_trial2_Urea_extraction.mgf	Mgf
	20190506_003_S196771_20190506_amnionUrea_D1C_trial2_Urea_extraction.pride.mgf.gz	Mgf
	20190506_003_S196771_20190506_amnionUrea_D1C_trial2_Urea_extraction.raw	Raw

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Publications

Comprehensive quantitative characterization of the human term amnion proteome.

Avilla-Royo Eva E Gegenschatz-Schmid Katharina K Grossmann Jonas J Kockmann Tobias T Zimmermann Roland R Snedeker Jess Gerrit JG Ochsenbein-Kölble Nicole N Ehrbar Martin M

Matrix biology plus 20210921

The loss of fetal membrane (FM) integrity and function at an early time point during pregnancy can have devastating consequences for the fetus and the newborn. However, biomaterials for preventive sealing and healing of FMs are currently non-existing, which can be partly attributed to the current fragmentary knowledge of FM biology. Despite recent advances in proteomics analysis, a robust and comprehensive description of the amnion proteome is currently lacking. Here, by an optimized protein sam ...[more]

PMID: 34765964

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