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The hTERT-RPE1 secretome after TLR3 stimulation

ABSTRACT: RPE cells perform a number of support functions in the inner eye including the secretion of signalling molecules and the maintenance of the immune privileged environment through communication with the immune system (Detrick and Hooks, 2010). Previous reports have demonstrated that RPE cells express a number of TLRs including the viral RNA receptor TLR3. To determine the complete secretory response of RPE cells downstream of poly(I:C) stimulation, we analysed conditioned medium from unstimulated or poly(I:C)-stimulated RPE cells using quantitative SILAC mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Htert-rpe1 Cell

SUBMITTER: Thomas O'Loughlin

LAB HEAD: Folma Buss

PROVIDER: PXD019445 | Pride | 2020-06-16

REPOSITORIES: Pride

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OPTN recruitment to a Golgi-proximal compartment regulates immune signalling and cytokine secretion.

O'Loughlin Thomas T Kruppa Antonina J AJ Ribeiro Andre L R ALR Edgar James R JR Ghannam Abdulaziz A Smith Andrew M AM Buss Folma F

Journal of cell science 20200615 12

Optineurin (OPTN) is a multifunctional protein involved in autophagy and secretion, as well as nuclear factor κB (NF-κB) and IRF3 signalling, and <i>OPTN</i> mutations are associated with several human diseases. Here, we show that, in response to viral RNA, OPTN translocates to foci in the perinuclear region, where it negatively regulates NF-κB and IRF3 signalling pathways and downstream pro-inflammatory cytokine secretion. These OPTN foci consist of a tight cluster of small membrane vesicles, w ...[more]

PMID: 32376785

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