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Synapse integrity and function: dependence on protein synthesis and identification of potential failure points

ABSTRACT: Synaptic integrity and function depend on myriad proteins - labile molecules with finite lifetimes that need to be continually replaced with freshly synthesized copies. Here we describe experiments designed to expose synaptic (and neuronal) properties and functions that are particularly sensitive to disruptions in protein supply, identify proteins lost early upon such disruptions, and uncover potential, yet currently underappreciated failure points. We report here that acute suppressions of protein synthesis are followed within hours by reductions in spontaneous network activity levels, impaired oxidative phosphorylation and mitochondrial function, reduced tenacity of both excitatory and inhibitory synapses and loss of synapses of both classes. Conversely, gross impairments in presynaptic vesicle recycling are observed only after several days, as is overt cell death. Proteomic analysis identified groups of potentially essential ‘early-lost’ proteins including regulators of synapse stability, proteins related to bioenergetics, fatty acid and lipid metabolism, and, unexpectedly, numerous proteins involved in Alzheimer’s disease pathology and amyloid beta processing.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Tamar Ziv

LAB HEAD: Professor Noam E. Ziv

PROVIDER: PXD037859 | Pride | 2022-11-27

REPOSITORIES: Pride

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