How legumes control nitrogen fixation by root nodule bacteria

ABSTRACT: Rhizobia are soil bacteria that can enter into complex symbiotic relationships with legumes, where rhizobia induce the formation of nodules on the plant root. Inside nodules, rhizobia differentiate into nitrogen-fixing bacteroids that reduce atmospheric nitrogen into ammonia, secreting it to the plant host in exchange for carbon. During the transition from free-living bacteria to bacteroids, rhizobial metabolism undergoes major changes. To investigate the metabolism of bacteroids and contrast it with the free-living state, we quantified the proteome of unlabelled bacteroids relative to 15N-labelled free-living rhizobia. The data were used to build a core metabolic model of pea bacteroids for the strain Rhizobium leguminosarum bv. viciae 3841.

INSTRUMENT(S):

ORGANISM(S): Rhizobium Leguminosarum Bv. Viciae 3841

TISSUE(S): Bacteroid

SUBMITTER: Gerhard Saalbach  

LAB HEAD: Philip Poole

PROVIDER: PXD019467 | Pride | 2021-09-09

REPOSITORIES: Pride