Extracellular membrane vesicle-driven crosstalk between legume plants and rhizobia: the symbiosome space as protein trafficking interface

ABSTRACT: Prokaryotes and eukaryotes secrete extracellular membrane vesicles (MVs) into the extracellular milieu to preserve and transport elevated concentrations of a given cargo across long distances. MVs encapsulate metabolites, DNA, RNA, and proteins, whose abundance and composition fluctuate depending on environmental cues. Importantly, MVs are involved in eukaryote-to-prokaryote communication owing to their ability to navigate different ecological niches and exchange molecular cargoes between the two domains. Amongst the different bacterium-host relationships, rhizobium-legume symbiosis is one of the closest known to nature. A crucial developmental stage of symbiosis is the formation of N2-fixing root nodules, which are endocytosed rhizobia -called bacteroids- confined by the plant-derived membrane. The unrestricted interface between the two membranes is the symbiosome space. To date many molecular aspects of symbiosis have been extensively studied, but the possibility of interbacterial and interdomain molecule trafficking by MVs in the symbiosome space was not questioned yet. Here we unveil intensive MV trafficking within the symbiosome interface of several rhizobium-legume dual systems by developing a robust MV isolation procedure. We analyse the MVs-encased proteomes encountered in the symbiosome space of each bacterium-host partnership uncovering both conserved and differential traits of every symbiotic system. This study opens the gates for designing MV-based biotechnological tools suitable for sustainable agriculture.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Lobelia Burttii Subsp. Burttii Sinorhizobium Fredii Hh103 Rhizobium Tropici Ciat 899

SUBMITTER: Lothar Jaensch  

LAB HEAD: Lothar Jaensch

PROVIDER: PXD056185 | Pride | 2025-05-07

REPOSITORIES: Pride