Proteomics

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Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate


ABSTRACT: Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S.haemolyticus virulence factors is scarce. Bacterial virulence is mediated by membrane vesicles (MVs) which enable secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the environment. We wanted to determine if the MV protein cargo of S.haemolyticus is strain specific and enriched in certain MV associated proteins compared to the totalsecretome. The present study shows that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. The MV cargo of both strains was enriched in proteins involved in adhesion and in acquisition of iron. The MV cargo of the clinical strain was further enriched in antimicrobial resistance proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus haemolyticus  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Jack-Ansgar Bruun  

LAB HEAD: Jorunn Pauline Cavanagh

PROVIDER: PXD010389 | Pride | 2018-11-30

REPOSITORIES: pride

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Publications

Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate.

Cavanagh Jorunn Pauline JP   Pain Maria M   Askarian Fatemeh F   Bruun Jack-Ansgar JA   Urbarova Ilona I   Wai Sun Nyunt SN   Schmidt Frank F   Johannessen Mona M  

Journal of proteomics 20181122


Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S. haemolyticus virulence factors is scarce. Bacterial membrane vesicles (MVs) are one mediator of virulence by enabling secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the e  ...[more]

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