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Interactome analysis of human RBMS1 protein in SW480 cells.

ABSTRACT: To identify protein binding partners of endogenous RBMS1, it was immunoprecipicated in triplicate, vs. IgG control, from SW480 colon adenocarcinoma cells, and subjected to MS analysis on an Q-exactive plus Orbitrap mass spectrometer. Raw files 13, 14, and 15 correspond to biological replicate IPs of RBMS1, and Raw files 16, 17, and 18 correspond to biological replicate IPs of IgG control.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Faraz Mardakheh

LAB HEAD: Faraz K. Mardakheh

PROVIDER: PXD019479 | Pride | 2020-06-10

REPOSITORIES: Pride

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RBMS1 Suppresses Colon Cancer Metastasis through Targeted Stabilization of Its mRNA Regulon.

Yu Johnny J Navickas Albertas A Asgharian Hosseinali H Culbertson Bruce B Fish Lisa L Garcia Kristle K Olegario John Paolo JP Dermit Maria M Dodel Martin M Hänisch Benjamin B Luo Yikai Y Weinberg Ethan M EM Dienstmann Rodrigo R Warren Robert S RS Mardakheh Faraz K FK Goodarzi Hani H

Cancer discovery 20200608 9

Identifying master regulators that drive pathologic gene expression is a key challenge in precision oncology. Here, we have developed an analytic framework, named PRADA, that identifies oncogenic RNA-binding proteins through the systematic detection of coordinated changes in their target regulons. Application of this approach to data collected from clinical samples, patient-derived xenografts, and cell line models of colon cancer metastasis revealed the RNA-binding protein RBMS1 as a suppressor ...[more]

PMID: 32513775

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