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New method to investigate protein orientation in corona of nanomaterials

ABSTRACT: The composition and structure of protein corona represents a crucial factor that controls the biological properties and fate of nanomaterials. While the composition of the corona can relatively easily be investigated by conventional bottom-up proteomics, assessing the spatial orientation of a protein in the corona (i.e assessing which epitopes are exposed on the outer surface) is still a challenging and time-consuming task. In this paper, we show that labeling the corona proteins with isobaric tags in their native conditions, and the subsequent analysis of MS/MS spectra of tryptic peptides, allows an easy and high-troughput assessment of the inner/outer orientation of the corresponding proteins in the original corona.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Andrea Armirotti

LAB HEAD: Andrea Armirotti

PROVIDER: PXD019524 | Pride | 2021-09-09

REPOSITORIES: Pride

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Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation.

Liessi Nara N Maragliano Luca L Castagnola Valentina V Bramini Mattia M Benfenati Fabio F Armirotti Andrea A

Analytical chemistry 20201207 2

The formation of the biomolecular corona represents a crucial factor in controlling the biological interactions and trafficking of nanomaterials. In this context, the availability of key epitopes exposed on the surface of the corona, and able to engage the biological machinery, is important to define the biological fate of the material. While the full biomolecular corona composition can be investigated by conventional bottom-up proteomics, the assessment of the spatial orientation of proteins in ...[more]

PMID: 33285070

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