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Global insights into lysine acetylome in Streptomyces coelicolor

ABSTRACT: By affinity enrichment using anti-acetyllysine antibody together with LC−MS/MS analysis, a total of 1,298 acetylation sites among 601 proteins were identified in S. coelicolor proteome

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Streptomyces Coelicolor

TISSUE(S): Cell Culture

SUBMITTER: Hong Zhang

LAB HEAD: Hong Zhang

PROVIDER: PXD019536 | Pride | 2021-09-30

REPOSITORIES: Pride

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Global Insights Into Lysine Acylomes Reveal Crosstalk Between Lysine Acetylation and Succinylation in Streptomyces coelicolor Metabolic Pathways.

Yang Yujiao Y Zhang Hong H Guo Zhenyang Z Zou Siwei S Long Fei F Wu Jiacheng J Li Peng P Zhao Guo-Ping GP Zhao Wei W

Molecular & cellular proteomics : MCP 20210914

Lysine acylations are reversible and ubiquitous post-translational modifications that play critical roles in regulating multiple cellular processes. In the current study, highly abundant and dynamic acetylation, besides succinylation, was uncovered in a soil bacterium, Streptomyces coelicolor. By affinity enrichment using anti-acetyl-lysine antibody and the following LC-MS/MS analysis, a total of 1298 acetylation sites among 601 proteins were identified. Bioinformatics analyses suggested that th ...[more]

PMID: 34530157

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2019-06-11 | PXD014202 | Pride

lysine acetylation and succinylation in Streptomyces coelicolor

Project description:To determine the acetylome of S. coelicolor, the wild-type S. coelicolor sample was prepared with three biological replicates and two technical replicates and analyzed. To quantify the acetylome and succinylome, three samples (wild-type, ∆SccobB1, and ∆SccobB2) with three biological replicates were prepared and analyzed. The cells were harvested, digested, and then subjected to affinity enrichment before LC-MS/MS analysis.

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The genome of Streptomyces rimosus shows a novel structure compared to other Streptomyces

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2024-01-26 | PXD041877 | Pride

Proteomic analysis of Streptomyces coelicolor vesicles

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Identification by ChIP-Seq of the binding sites of the extracytoplasmic function (ECF) sigma factor SigE in the genome of Streptomyces coelicolor A3(2) M600.

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