Proteomics

Dataset Information

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Recombinant Tau and EFhd2 from Bacteria

ABSTRACT: E. coli is a commonly used host for generating large quantities of human recombinant proteins. These proteins are often considered free of post-translational modifications (PTMs); however, a subset of the produced proteins can incorporate modifications derived from the host bacterial enzymes. Here, we used tandem mass spectrometry to identify inadvertent PTMs on human recombinant proteins produced in E. coli. As proof of concept, we identified bacteria-mediated PTMs on human tau and EFhd2 proteins, which we used in in vitro studies to understand their role in Alzheimer's disease (AD). Some of the identified PTMs have been previously associated with AD while others are novel. Additionally, we identified E. coli proteins that co-purified with these human recombinant proteins even after several purification steps.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Cell Culture

DISEASE(S): Frontotemporal Dementia,Alzheimer's Disease

SUBMITTER: Jared Lamp  

LAB HEAD: Irving Vega

PROVIDER: PXD020022 | Pride | 2025-07-08

REPOSITORIES: Pride

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190328-EFHD2-1.raw Raw
190328-EFHD2-2.raw Raw
190328-EFHD2-3.raw Raw
190328-EFHD2-ImprovedBacteriaDB.mzML Mzml
190328-EFHD2-ImprovedBacteriaDB.mzid.gz Mzid
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Publications

Validation of recombinant human protein purified from bacteria: An important step to increase scientific rigor.

Umstead Andrew A   Soliman Ahlam S AS   Lamp Jared J   Vega Irving E IE  

Analytical biochemistry 20201021

E. coli is a common host for generating human recombinant proteins in in vitro studies that seek to understand the biochemical and structural properties of proteins and in drug discovery. Validation of this biological resource is crucial to avoid misinterpretations and assay interference. Here, we demonstrate the use of tandem mass spectrometry to detect inadvertent post-translational modifications on human recombinant proteins produced in E. coli. Additionally, we identified co-purified E. coli  ...[more]

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