Project description:We investigated the composition of the interactomes of overall 7 proteins in the parasitic protist Giardia lamblia, some of which were found to be associated to clathrin assemblies. This accession refers to proteins GlPXD2 and 4
Project description:We investigated the composition of the interactomes of 7 proteins in the parasitic protist Giardia lamblia, some of which were found to be associated to clathrin assemblies. This accession refers GlNECAP.
Project description:We investigated the composition of the interactomes of 7 proteins in the parasitic protist Giardia lamblia, some of which were found to be associated to clathrin assemblies. This accession refers to the first three namely GlPXD1, 3 and 6.
Project description:We investigated the composition of the interactomes of 7 proteins in the parasitic protist Giardia lamblia, some of which were found to be associated to clathrin assemblies. This accession refers to GlFYVE.
Project description:Serine residues in the charged linker region of human Hsp90beta are highly phoshporylated in vivo. Mutation of these residues to alanines resulted in altered binding of many Hsp90beta interactors. This project contains the data and searches related to co-immunoprecipitation experiments with wild-type and mutant Hsp90beta, as well as input.
Project description:This protocol describes a methodology combining TurboID - a recently developed proximity biotinylation technique - with conventional epitope-tag based co-immunoprecipitation (Co-IP) to analyse protein-protein interactions (PPIs) in cell culture systems. This integrated approach allows for the targeted examination of both transient and stable protein interactors, enhancing our understanding of protein dynamics. TurboID captures transient interactions often missed by Co-IP, which captures high aCinity, stable interactors. Combination of both techniques enables direct comparison of interaction strengths, providing insights into the dynamic nature of protein interactions within cells. The rapid biotinylation capability of TurboID reduces background noise and false positives while Co- IP enriches stable interactors, together improving data quality and interpretation of the interactomic landscape. Demonstrating the eCicacy of this methodology, proteins relevant to the pathology of Spinal Muscular Atrophy were utilised to explore variations at the interactome level. The use of identical starting cell lysates for both TurboID and Co-IP minimised variability and ensured datasets were comparable, allowing for consistency and enhancing the reliability of findings regarding the nature and strength of protein interactions. This novel framework eCectively combines both innovative and classical techniques while maintaining consistency in sample handling, advancing our understanding of the intricate networks that govern cellular processes.
Project description:This study describes the epigenetic profiling of the novel interactors of H3K4me3, H3K36me3 or H3K9me3. The interactors were ChIP-Seq profiled by their GFP tag in stably transfected HeLa (Kyoto) cells. The interactors include GATAD1, Sgf29, BAP18, TRRAP, PHF8, N-PAC and LRWD1 (including replicates), as well as an GFP ChIP-Seq profile on non-transfected HeLa cells (negative control). Also included are the profiles of the histone modifications themselves (H3K4me3, H3K27me3, H3K9me3, H3K36me3, H3K9/14Ac and H3K79me3) ChIP-Seq profiling of 8 proteins by their GFP tag in stably transfected cells HeLa (Kyoto) cells, 6 replicas, as well as ChIP-Seq profiling of 6 histone modifications in wt HeLa (Kyoto) cells
Project description:To access ESCRT subunit presence, distribution and main interactors, we performed iterative co-immunoprecipitation on recombinant Giardia subunits.
Project description:ATP-dependent chromatin remodeling enzymes re-position and evict nucleosomes at specific genomic loci and therefore are essential regulators of all DNA dependent processes. They act in the context of large multiprotein complexes. The malaria-causing parasite Plasmodium falciparum (Pf) possesses a reduced set of chromatin remodeling enzymes, which are highly divergent, and no associated complex subunits are known. Within a detailed characterization of the ISWI-type remodeler PfSnf2L (PF3D7_1104200), potential interactors were identified. The protein was tagged endogenously in the parasite and co-immunoprecipitated. Subsequent LC-MS/MS analysis provided a list of specific protein interactors with a strong link to chromatin organization including nucleosome assembly factors, transcription factors as well as Plasmodium-specific uncharacterized proteins. The results suggest a functional role of PfSnf2L in nucleosome assembly and transcription regulation and point towards novel Plasmodium-specific chromatin remodeling complexes.
Project description:To analyze OTX2 function in adult choroid plexus, we performed several OTX2 co-immunoprecipitation (co-IP) experiments with mass spectrometry analysis to identify potential protein partners. We previously discovered that OTX2 protein also accumulates non-cell autonomously in subventricular zone (SVZ) and rostral migratory stream (RMS) astrocytes and in visual cortex (VCx) parvalbumin cells. The identification of alternate protein partners in cell-autonomous and non-cell-autonomous contexts would suggest OTX2 takes on specific roles after transferring between cells. In order to test this hypothesis, and to reinforce choroid plexus analysis, we also performed OTX2 co-IP on lysates from adult mouse SVZ, RMS and VCx.