Phosphoproteome of five-minute KCl-depolarised mouse cultured hippocampal neurons

ABSTRACT: Cultured hippocampal neurons from mice were treated with high concentration KCl for five minutes to depolarise the plasma membrane and model sustained brain activity. A high KCl condition was compared to low KCl using phosphoproteomics. Phosphopeptides were enriched from trypsin digested bio-replicates of the high and low KCl conditions and analysed by mass spectrometry. Approximately 20,000 phosphopeptides were identified enabling a comparison of the relative level of phosphorylation in response to depolarisation.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Neuron, Hippocampal Neuron, Hippocampus

SUBMITTER: Mark Graham  

LAB HEAD: Mark Evan Graham

PROVIDER: PXD020232 | Pride | 2023-10-15

REPOSITORIES: Pride