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Phosphoproteome of five-minute KCl-depolarised mouse cultured hippocampal neurons

ABSTRACT: Cultured hippocampal neurons from mice were treated with high concentration KCl for five minutes to depolarise the plasma membrane and model sustained brain activity. A high KCl condition was compared to low KCl using phosphoproteomics. Phosphopeptides were enriched from trypsin digested bio-replicates of the high and low KCl conditions and analysed by mass spectrometry. Approximately 20,000 phosphopeptides were identified enabling a comparison of the relative level of phosphorylation in response to depolarisation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Neuron, Hippocampal Neuron, Hippocampus

SUBMITTER: Mark Graham

LAB HEAD: Mark Evan Graham

PROVIDER: PXD020232 | Pride | 2023-10-15

REPOSITORIES: Pride

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Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles.

Longfield Shanley F SF Mollazade Mahdie M Wallis Tristan P TP Gormal Rachel S RS Joensuu Merja M Wark Jesse R JR van Waardenberg Ashley J AJ Small Christopher C Graham Mark E ME Meunier Frédéric A FA Martínez-Mármol Ramón R

Nature communications 20231110 1

Neuronal communication relies on the release of neurotransmitters from various populations of synaptic vesicles. Despite displaying vastly different release probabilities and mobilities, the reserve and recycling pool of vesicles co-exist within a single cluster suggesting that small synaptic biomolecular condensates could regulate their nanoscale distribution. Here, we performed a large-scale activity-dependent phosphoproteome analysis of hippocampal neurons in vitro and identified Tau as a hig ...[more]

PMID: 37949856

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