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Innate and adaptive serum proteomes of Santa Gertrudis cattle in response to Rhipicephalus microplus infestation

ABSTRACT: Previous studies have applied genomics and transcriptomics to identify the immune and genetic markers as key indicator traits for cattle tick susceptibility/resistance, however, results differed between breeds, and host proteomics and metabolomics were not considered. We used serum samples from Santa Gertrudis cattle phenotypically divided into groups as tick-resistant (TR) and -susceptible (TS) to conduct differential abundance analyses of protein profiles between the two groups. The serum proteins were digested into peptides followed by identification and quantification by sequential window acquisition of all instances of theoretical fragment ion mass spectrometry (SWATH-MS).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Ali Raza

LAB HEAD: Ala Tabor

PROVIDER: PXD020518 | Pride | 2024-05-10

REPOSITORIES: Pride

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Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks.

Raza Ali A Schulz Benjamin L BL Nouwens Amanda A Jackson Lousie A LA Piper Emily K EK James Peter P Jonsson Nicholas N NN Tabor Ala E AE

Parasite immunology 20210411 7

Previous studies have applied genomics and transcriptomics to identify immune and genetic markers as key indicator traits for cattle tick susceptibility/resistance; however, results differed between breeds, and there is lack of information on the use of host proteomics. Serum samples from Santa Gertrudis cattle (naïve and phenotyped over 105 days as tick-resistant [TR] or tick-susceptible [TS]) were used to conduct differential abundance analyses of protein profiles. Serum proteins were digested ...[more]

PMID: 33843060

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