Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Vicia narbonensis (legume) and Pisum sativum (pea) expressing a bacterial phosphoenol pyruvate carboxylase during seed maturation

ABSTRACT: In order to change assimilate partitioning during seed maturation a bacterial phosphoenolpyruvate carboxylase (PEPC) has been expressed in V. narbonensis under control of the LegB4 promoter. Transgenic bean seeds accumulate app. 20% more protein and have higher seed weight. Physiological and biochemical analyses indicate a shift of metabolic fluxes from sugars/starch to organic acids/free amino acids suggesting increased anaplerotic carbon flow. This indicates that the availability of carbon acceptors limits seed protein synthesis. Gene expression was analysed in growing embryos using macro-arrays containing 5,548 seed-specific pea genes. Radioactive labeled cDNA probes were prepared from RNA isolated from embryo of developing seeds of wild type and transgenic PEPC plants of V. narbonensis (15, 17, 19, 21, 25, and 30 DAP) and hybridized to cDNA macroarrays.

ORGANISM(S): Pisum sativum

SUBMITTER: Ruslana Radchuk

PROVIDER: E-IPKG-2 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of pea seed development

Project description:SnRK1 (sucrose-non-fermenting-1-related) protein kinases are involved in the regulation of plant metabolism controlling both gene expression and phosphorylation. The aim of the study was to investigate the role of SnRK1 in pea seed development. To study the effect of SnRK1 deficiency, transgenic pea plants were generated carrying a gene for VfSnRK1 in antisense orientation under control of seed specific vicilin promoter. Selected transgenic lines were characterized with decreased levels of PsSnRK1 mRNA and reduced up to 71% phosphorylation activity. Antisense inhibition of SnRK1 resulted in reduced seeds fresh weight, defect of pollen development. To dissect the SnRK1-antisense phenotype at the molecular level, a search for genes with differential expression patterns in transgenic plant versus wild type seeds has been performed using cDNA macroarray analysis. Radioactive labeled cDNA probes were prepared from RNA isolated from embryo of developing seeds of wild type (11, 13, 15, 17, 19, and 21 DAP) and transgenic SnRK1-antisense plant (13, 15, 17 and 19 DAP), which correspond to the transition phase of seed development, and hybridized to cDNA macroarrays.

2013-01-30 | E-IPKG-1 | biostudies-arrayexpress

Transcription profiling of fusiform cambial and ray cambial cells from poplar trees

Project description:(paper abstract) Wood is a renewable resource that serves as a sustainable raw material for energy, fuels and materials. A basic understanding of the cambial meristem and the origin of wood cells is critical for forest tree molecular breeding to increase the availability of woody raw materials. Gene expression per cell type in the cambium of poplar (Populus trichocarpa x P. deltoïdes cultivar Boelare) was investigated using poplar microarray. Cells were hand microdissected from lyophilized sections of vascular cambial zone sampled in growing poplar. Two cell types were isolated, fusiform cambial cells (FCCs) and ray cambial cells (RCCs). Results showed about one thousand genes differentially expressed per cell type. Microarray expression results were validated by a second cell sampling used for reverse northern dot blot and RT-PCR analysis. RCCs revealed specific photosynthetic competences involving at least 35 genes of the photosystems. RCCs revealed also a marked difference for intercellular transport involving notably aquaporin genes. Distinct classes of cell-wall related genes were detected in RCCs and FCCs. A candidate xyloglucan endotransglycosylase showed to be upregulated and highly expressed in FCCs and an extensin family gene was also very highly expressed in these cells. A candidate polygalacturonase was strikingly expressed specifically in RCCs. A marked expression of cytoskeleton genes was also reported for FCCs where profilin family genes were very highly expressed. Related to cell signaling and regulation, one of the most intriguing result was the observation of LAX1 gene specificity in FCCs whereas PIN genes were expressed in both cell types.

2008-06-30 | E-MEXP-794 | biostudies-arrayexpress

Transcription profiling of wild type and jasmonate deficient mutant aos Arabidopsis plants in control conditions

Project description:comparison of the transcriptome of Columbia WT and the jasmonate deficient mutant aos (allene oxide synthase)in control conditions.

2008-02-01 | E-ATMX-36 | biostudies-arrayexpress

Transcription profiling of wild type and trienoic fatty acid deficient mutant fad3-2/fad7-2/fad8 Arabidopsis plants in control conditions

Project description:comparison of the transcriptome of Columbia-0 WT and the trienoic fatty acid deficient mutant fad3-2/fad7-2/fad8 in control conditions.

2008-02-01 | E-ATMX-37 | biostudies-arrayexpress

Transcription profiling of wounded leaves from Arabidopsis wild type and jasmonic acid mutants

Project description:Arabidopsis leaves wounded for 1 hr, comparison of jasmonic acid (JA) mutants

2007-04-18 | E-ATMX-9 | biostudies-arrayexpress

Chromatin immunoprecipitation genome wide binding site identification in yeast

Project description:Genome Wide Location: binding site identification

2013-01-30 | E-WMIT-1 | biostudies-arrayexpress

Triticum turgidum-Triticum boeoticum amphiploids UPLC–Orbitrap-MS

Project description:This data set extends the original data from the UPLC–Orbitrap-MS portion of this article:Molecular cytogenetic identification and nutritional composition evaluation of newly synthesized Triticum turgidum-Triticum boeoticum amphiploids (AABBAbAb)

2023-11-28 | PXD046077 | Pride

Transcription profiling of prostates from normal and Pten knock-out mice, and comparing the gene expression profile in the proximal and distal part of normal prostates

Project description:1. Comparison of gene expression profiles of normal prostates and prostates isolated from 4-5 months old PSA-Cre;Pten-loxP/loxP mice<br>2.Comparison of the gene expression profile in the proximal and distal part of normal prostates

2009-08-07 | E-MEXP-2029 | biostudies-arrayexpress

Transcription profiling of synchronized Sulfolobus acidocaldarius populations as they progress through the cell cycle

Project description:Exponentially growing Sulfolobus acidocaldarius were treated with NaAc to generate replication runout and arrest in G2 phase. The cells were then resuspended in fresh acetate-free media which generates a synchronous population. Samples for investigation of gene expression change were taken during the synchronised populations progress through the cell cycle.

2007-12-08 | E-MEXP-936 | biostudies-arrayexpress

Transcription profiling of reference RNA samples using automated cDNA sysnthesis and labeling to investigate effect on reproducibility

Project description: Not available

2009-08-17 | E-TABM-749 | biostudies-arrayexpress