Proteomics

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Mouse cerebellum LC-MSMS: effect of calorie restriction

ABSTRACT: The cerebellum harbors a circadian clock that can be shifted by scheduled mealtime and participates in behavioral anticipation of food access. To determine which cerebellar proteins are modified by time-of-day and/or feeding time, we determined day-night variations of proteome in the cerebellum of mice fed either ad libitum or only during daytime (from noon to lights off). Two-dimensional differences in gel electrophoresis (2D-DIGE) combined with two-way analyses of variance reveals that a majority of cerebellar proteins are significantly regulated by feeding conditions (food availability). Levels of few other cerebellar proteins were modulated exclusively by daily (or circadian) cues, independent of meal time, and others due to combined influence of meal time and time-of-day. Changes reflect behavioral anticipation of mealtime and/or feeding-induced shift in the circadian clock of the cerebellum.

INSTRUMENT(S): Synapt HDMS, maXis 4G

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebellum

SUBMITTER: Fabrice BERTILE  

LAB HEAD: Sarah Cianférani

PROVIDER: PXD021056 | Pride | 2021-04-06

REPOSITORIES: Pride

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Publications

Daytime Restricted Feeding Affects Day-Night Variations in Mouse Cerebellar Proteome.

Bertile Fabrice F   Plumel Marine M   Maes Pauline P   Hirschler Aurélie A   Challet Etienne E  

Frontiers in molecular neuroscience 20210412

The cerebellum harbors a circadian clock that can be shifted by scheduled mealtime and participates in behavioral anticipation of food access. Large-scale two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry was used to identify day-night variations in the cerebellar proteome of mice fed either during daytime or nighttime. Experimental conditions led to modified expression of 89 cerebellar proteins contained in 63 protein spots. Five and 33 spots were changed  ...[more]

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