T7 DNA Polymerase with fluorescent unnatural amino acid

ABSTRACT: A T7 DNA polymerase variant with the fluorescent unnatural amino acid (7-hydroxycoumarin-4-yl) ethylglycine (7-HCou) incorporated at position 514 was expressed in E. coli and purified. The protein was then characterized by mass spec, pre-steady state kinetic experiments, and fluorescence measurements.

INSTRUMENT(S):

ORGANISM(S): Escherichia Virus T7

SUBMITTER: Tyler Dangerfield  

LAB HEAD: Kenneth A. Johnson

PROVIDER: PXD021676 | Pride | 2020-10-19

REPOSITORIES: Pride