Proteomics

Dataset Information

1

Structure of the human signal peptidase complex


ABSTRACT: The ER-resident signal peptidase complex (SPC) is an essential membrane protein complex involved in the maturation of soluble and type-I transmembrane proteins destined to the secretory pathway. As a part of the ER translocon machinery, the SPC removes signal peptides (SPs) from the N-termini of many proteins such as hormones, antibodies, or secretory enzymes1,2. To accomplish this task, the SPC must process a large variety of SPs with an exquisite specificity. Although the determinants of SP recognition have been established empirically3, the molecular details of SP recognition and removal remain elusive. Here, we show that the human SPC exists in two functional paralogs with distinct proteolytic subunits. We determined the structures and posttranslational modifications of both paralogs using electron cryo-microscopy and structural proteomics. The respective proteolytic subunits SEC11A and SEC11C adopt a conserved signal peptidase (SPase) fold with their active sites facing a prominent transmembrane window, which is collectively formed by the transmembrane domains of all SPC subunits. Characteristic, charged regions on both sides of this window locally thin the membrane by approximately 40% compared to the exterior. The data suggest a mechanistic model for SPC specificity and the transfer of SPs from the Sec61 translocation channel to the SPC.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo sapiens   Bos taurus  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Barbara Steigenberger  

LAB HEAD: Richard A. Scheltema

PROVIDER: PXD022002 | Pride | 2021-07-05

REPOSITORIES: Pride

Similar Datasets

2019-08-20 | PXD013769 | Pride
2021-07-02 | PXD024160 | Pride
2020-03-19 | PXD017382 | Pride
2021-07-08 | PXD023072 | Pride
2020-07-22 | PXD018189 | Pride
2020-05-11 | PXD015941 | Pride
2021-05-28 | PXD022675 | Pride
1000-01-01 | S-EPMC2748100 | BioStudies
2018-07-23 | PXD007513 | Pride
2019-04-03 | PXD010796 | Pride