Dataset Information


Cross-linking mass spectrometry of the CCAN and CCAN-Cenp-A complexes

ABSTRACT: Here we performed a cross-linking mass spectrometry analysis of the inner kinethocore complex CCAN and of the complex associated with a CenpA-nucleosome (CCAN-CenpA)from budding yeast. These experiments validated the cryo-EM structure of both complexes and provided additional structural insights on the complex organization.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces cerevisiae  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Domenico Fasci  

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD013769 | Pride | 2019-08-20


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Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.

Yan Kaige K   Yang Jing J   Zhang Ziguo Z   McLaughlin Stephen H SH   Chang Leifu L   Fasci Domenico D   Ehrenhofer-Murray Ann E AE   Heck Albert J R AJR   Barford David D  

Nature 20191002 7777

In eukaryotes, accurate chromosome segregation in mitosis and meiosis maintains genome stability and prevents aneuploidy. Kinetochores are large protein complexes that, by assembling onto specialized Cenp-A nucleosomes1,2, function to connect centromeric chromatin to microtubules of the mitotic spindle3,4. Whereas the centromeres of vertebrate chromosomes comprise millions of DNA base pairs and attach to multiple microtubules, the simple point centromeres of budding yeast are connected to indivi  ...[more]

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