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Cross-linking mass spectrometry of the CCAN and CCAN-Cenp-A complexes

ABSTRACT: Here we performed a cross-linking mass spectrometry analysis of the inner kinethocore complex CCAN and of the complex associated with a CenpA-nucleosome (CCAN-CenpA)from budding yeast. These experiments validated the cryo-EM structure of both complexes and provided additional structural insights on the complex organization.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Domenico Fasci

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD013769 | Pride | 2019-08-20

REPOSITORIES: Pride

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Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.

Yan Kaige K Yang Jing J Zhang Ziguo Z McLaughlin Stephen H SH Chang Leifu L Fasci Domenico D Ehrenhofer-Murray Ann E AE Heck Albert J R AJR Barford David D

Nature 20191002 7777

In eukaryotes, accurate chromosome segregation in mitosis and meiosis maintains genome stability and prevents aneuploidy. Kinetochores are large protein complexes that, by assembling onto specialized Cenp-A nucleosomes<sup>1,2</sup>, function to connect centromeric chromatin to microtubules of the mitotic spindle<sup>3,4</sup>. Whereas the centromeres of vertebrate chromosomes comprise millions of DNA base pairs and attach to multiple microtubules, the simple point centromeres of budding yeast a ...[more]

PMID: 31578520

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