Dataset Information

Proteome of Listeria monocytogenes FBUNT biofilm cells and response to bacteriocin AL705

ABSTRACT: L. monocytogenes FBUNT is able to form biofilm after six days at 10 ºC. Proteome under planktonic growth, lactocin AL705-treated and untreated biofilms was analyzed. Compared to planktonic cells, protein abundance shifts associated with ribosomes function and biogenesis, cell membrane functionality, carbohydrate and amino acid metabolism and transport were observed in sessile cells. When sessile cells were treated with lactocin AL705, proteins up-regulation were mostly related to carbohydrate metabolism and nutrient transport in an attempt to compensate for impaired energy generation caused by bacteriocin interacting with the cytoplasmic membrane. Notably, transport systems as β-glucosidase IIABC (lmo0027), cellobiose (lmo2763) and trehalose (lmo1255) specific PTS proteins were highly overexpressed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Listeria Monocytogenes Serotype 4b Str. 02-6679

SUBMITTER: Lucia Mendoza

LAB HEAD: Lucia Mendoza

PROVIDER: PXD022168 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Proteomic Analysis of Listeria monocytogenes FBUNT During Biofilm Formation at 10°C in Response to Lactocin AL705.

Melian Constanza C Castellano Patricia P Segli Franco F Mendoza Lucía M LM Vignolo Graciela Margarita GM

Frontiers in microbiology 20210129

Listeria monocytogenes is one of the major food-related pathogens and is able to survive and multiply under different stress conditions. Its persistence in industrial premises and foods is partially due to its ability to form biofilm. Thus, as a natural strategy to overcome L. monocytogenes biofilm formation, the treatment with lactocin AL705 using a sublethal dose (20AU/ml) was explored. The effect of the presence of the bacteriocin on the biofilm formation at 10°C of L. monocy ...[more]

PMID: 33584610

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Project description:Otitis media, pneumonia, sinusitis and as well as severe diseases such as meningitis and bacteraemia are related to biofilm-like diseases, in which Streptococcus pneumoniae demonstrated differential and tissue specific gene expressions. In this study, we reported the differential gene expression profile of early in vitro biofilm and planktonic cell in c-DNA microarray analysis. The microarray analysis was performed on total RNA extracted from biofilms grown in 24-well microtiter plate and mid-log grown planktonic cells. To validate the results of microarray, real-time RT-PCR was performed on 13 differentially expressed genes and one constitutively expressed gene from six different functional groups. cDNA-microarray analyses indicated 89 genes that were significantly differentially expressed in biofilm and planktonic cells. Among total differentially expressed gene, almost 50% were hypothetical genes. Of the 46 protein coding genes, 34 showed up-regulation and 16 showed down-regulation in biofilm. The functional annotation showed that many functional categories were differentially regulated in biofilm and planktonic cells, such as genes involve in purine, pyrimidine nucleotide metabolism, RNA/DNA metabolism, amino acid transport and metabolism, translation, transporter protein, carbohydrate transport and metabolism, cell wall biosynthesis, isoprenoid biosynthesis, transcription regulator and cellular process. Streptococcus pneumoniae R6 strain used in this is an unencapsulated and avirulent strain derived from encapsulated serotype 2 pathogenic strain D39. In vitro biofilm formation was carried out in 24-well, flat-bottom, polystyrene microtiter plate (BD falcon, MD, USA) in static model. S. pneumoniae grown up to mid-logarithmic phase in TSB medium was diluted 1:100 with fresh sterile TSB medium supplied with 1% glucose, inoculated 1.5 mL in 24-well microtiter plate and, incubated for 15 hours at 37M-BM-0C in 5% CO2. After incubation medium was discarded, and the plates were gently washed three times with 1.5 mL sterile, cold phosphate buffer saline (PBS). Adherent cell were scraped and immediately processed for RNA extraction. For planktonic cells RNA extraction, five ml of mid-logarithmic phase cell suspension was pelleted by centrifugation and wash three times with sterile PBS and immediately processed for RNA extraction. All experiments were performed in triplicate (3 independent biological replicates)

Dataset Information

Proteome of Listeria monocytogenes FBUNT biofilm cells and response to bacteriocin AL705

Publications

Proteomic Analysis of <i>Listeria monocytogenes</i> FBUNT During Biofilm Formation at 10°C in Response to Lactocin AL705.

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