Proteomics

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Quantitative proteomic TandemMassTag analysis of intestinal epithelial cells (IEC) deleted for both Hdac1 and Hdac2


ABSTRACT: We have performed quantitative proteomic TandemMassTag to investigate proteomic changes after deletion of epigenetic eraser genes Hdac1 and Hdac2 in intestinal epithelial cells. Both HDAC1 and HDAC2 are epigenetic erasers that drive specific and redundant gene expression patterns, in part by removing acetyl groups on histones. Deletion of these Hdac in intestinal epithelial cell (IEC) in vivo alters intestinal homeostasis, dependent on the Hdac deleted and the level of expression of both. To determine the specific IEC function of HDAC1 and HDAC2, we have performed transcriptomic and quantitative proteomic approaches on IEC deficient in Hdac1 and Hdac2. We have defined changes in both mRNA and protein expression patterns affecting IEC differentiation. We have identified IEC Hdac1- and Hdac2-dependent common as well as specific pathways and biological processes. These findings uncover unrecognized similarities and differences between Hdac1 and Hdac2 in IEC.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Small Intestine Epithelium

DISEASE(S): Not Available

SUBMITTER: Dominique Levesque  

LAB HEAD: Claude Asselin

PROVIDER: PXD022558 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

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Publications

JAK-STAT Pathway Inhibition Partially Restores Intestinal Homeostasis in <i>Hdac1</i>- and <i>Hdac2</i>-Intestinal Epithelial Cell-Deficient Mice.

Gonneaud Alexis A   Turgeon Naomie N   Boisvert Francois-Michel FM   Boudreau Francois F   Asselin Claude C  

Cells 20210123 2


We have previously reported that histone deacetylase epigenetic regulator <i>Hdac1</i> and <i>Hdac2</i> deletion in intestinal epithelial cells (IEC) disrupts mucosal tissue architecture and barrier, causing chronic inflammation. In this study, proteome and transcriptome analysis revealed the importance of signaling pathways induced upon genetic IEC-<i>Hdac1</i> and <i>Hdac2</i> deletion. Indeed, Gene Ontology biological process analysis of enriched deficient IEC RNA and proteins identified comm  ...[more]

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