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The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast function

ABSTRACT: Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR expression are poorly understood. Here we show that the Arabidopsis NOT4A ubiquitin-ligase positively regulates PROTON GRADIENT 3 (PGR3), a PPR protein required for translating 30S ribosome subunits and several thylakoid-localised photosynthetic components within chloroplasts.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf, Shoot

SUBMITTER: Sacha Baginsky

LAB HEAD: Sacha Baginsky

PROVIDER: PXD022584 | Pride | 2021-09-09

REPOSITORIES: Pride

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The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation.

Bailey Mark M Ivanauskaite Aiste A Grimmer Julia J Akintewe Oluwatunmise O Payne Adrienne C AC Osborne Rory R Labandera Anne-Marie AM Etherington Ross D RD Rantala Marjaana M Baginsky Sacha S Mulo Paula P Gibbs Daniel J DJ

Nature communications 20210111 1

Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR expression are poorly understood. Here we show that the Arabidopsis NOT4A ubiquitin-ligase positively regulates the expression of PROTON GRADIENT REGULATION 3 (PGR3), a PPR protein required for trans ...[more]

PMID: 33431870

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