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Quantitative proteomic analysis reveals altered enzyme expression profile in Zea mays roots during the early stage of colonization by Herbaspirillum seropedicae

ABSTRACT: The use of plant growth-promoting bacteria as agricultural inoculants of plants should be encouraged because of their prominent role in biological nitrogen fixation, an increase of nutrient uptake by roots, abiotic stress mitigation, and disease control. The complex underlined mechanisms of plant and beneficial bacteria association have been increasingly studied and proteomic tools can expand our perception about fundamental molecular processes modulated by the interaction. In this study, we investigated the changes in protein expression in maize roots in response to treatment with the endophytic diazotroph Herbaspirillum seropedicae. To identify maize proteins whose expression levels were altered in the presence of bacteria, a label-free quantitative proteomic approach was used. Using this approach, we identified 123 differentially expressed proteins, of which 34 were upregulated enzymes, in maize roots cultivated with H. seropedicae. The maize root colonization of H. seropedicae modulated the differential expression of enzymes involved in the stress response and activity of enzymes related to nitrogen metabolism.

INSTRUMENT(S):

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Root

SUBMITTER: Proteomics Unit

LAB HEAD: Fabio Nogueira

PROVIDER: PXD022617 | Pride | 2026-07-29

REPOSITORIES: Pride

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			Action	DRS
	B1.sepr	Other
	B2.sepr	Other
	B3.sepr	Other
	C1.sepr	Other
	C2.sepr	Other

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Quantitative proteomic analysis reveals altered enzyme expression profile in Zea mays roots during the early stage of colonization by Herbaspirillum seropedicae

Project description:It was investigated the changes in protein expression in maize roots in response to treatment with Herbaspirillum seropedicae. To identify maize proteins whose expression levels were altered in the presence of bacteria, a label-free quantitative proteomic approach was used.

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Aberrant astrocyte protein secretion contributes to altered neuronal development in diverse disorders

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Zea mays under 24 h hypoxia - shotgun of soluble proteins

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Mouse inguinal white adipose tissue (iWAT) LC-MS

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Evaluation of the secretome of senescent melanoma cells and the impact of silencing Mfn1 on the senescence-associated secretory phenotype (SASP) by shotgun proteomics analysis.

Project description:Cellular senescence is a therapy endpoint in melanoma, and the senescence-associated secretory phenotype (SASP) can affect tumor growth and microenvironment, influencing treatment outcomes. Metabolic interventions can modulate the SASP, and an enhanced mitochondrial energy metabolism supports resistance to therapy in melanoma. In a previous report we showed that in melanoma, senescence induced by the DNA methylating agent temozolomide, increases fusion proteins mitofusins 1 and 2. Silencing Mfn1 or Mfn2 expression reduced interleukin-6 secretion by senescent cells. Here we expanded these observations evaluating the secretome of senescent melanoma cells using shotgun proteomics, and explored the impact of silencing Mfn1 on the SASP. A significant increase in proteins reported to reduce the immune response towards the tumor was found in the media of senescent cells. The secretion of several of these immunomodulatory proteins was affected by Mfn1 silencing, among them was galectin-9. In agreement, tumors lacking mitofusin 1 responded better to treatment with the methylating agent dacarbazine, tumor size was reduced and a higher immune cell infiltration was detected in the tumor. Our results highlight mitochondrial dynamic proteins as potential pharmacological targets to modulate the SASP in the context of melanoma treatment.

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EVALUATION OF THE EFFECTS OF HUMIC ACIDS ON MAIZE ROOT ARCHITECTURE BY LABEL-FREE PROTEOMICS ANALYSIS

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Identification of factors involved in the adaptation to superoxide stress in pathogenic Leptospira

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LC-MS/MS proteomic analysis of pharyngeal swabs and plasma from MHV-infected and uninfected mice

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2024-12-11 | PXD054355 | Pride

Arabidopsis thaliana roots: inoculated roots with a bacterium Antarctic (Pseudomonas sp, Dac-bac TI-8) vs untreated roots (control)

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Gené organ engagement in lipid metabolism revealed by a combined transcriptomic and proteomic approach

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