Dataset Information

Sickle cell trait modulates the proteome and phosphoproteome of Plasmodium falciparum-infected erythrocyte

ABSTRACT: The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the erythrocyte surface that interact with key human proteins in the Ankyrin R and 4.1R complexes. Oxidative stress generated by HbS, as well as by parasite invasion, disrupts the kinase/phosphatase balance, potentially interfering with the molecular interactions between human and parasite proteins. HbS is known to be associated with abnormal membrane display of parasite antigens. Studying the proteome and the phosphoproteome of red cell membrane extracts from P. falciparum infected and non-infected erythrocytes, we show here that HbS heterozygous carriage, combined with infection, modulates the phosphorylation of erythrocyte membrane transporters and skeletal proteins as well as of parasite proteins. Our results highlight modifications of Ser- /Thr- and/or Tyr- phosphorylation in key human proteins, such as ankyrin, β-adducin, β-spectrin and Band 3, and key parasite proteins, such as RESA or MESA. Altered phosphorylation patterns could disturb the interactions within membrane protein complexes, affect nutrient uptake and the infected erythrocyte cytoadherence phenomenon, thus lessening the severity of malaria symptoms.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human) Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Erythrocyte

SUBMITTER: Cerina Chhuon

LAB HEAD: Anaïs Merckx

PROVIDER: PXD023280 | Pride | 2021-04-19

REPOSITORIES: Pride

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Publications

Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes.

Chauvet Margaux M Chhuon Cerina C Lipecka Joanna J Dechavanne Sébastien S Dechavanne Célia C Lohezic Murielle M Ortalli Margherita M Pineau Damien D Ribeil Jean-Antoine JA Manceau Sandra S Le Van Kim Caroline C Luty Adrian J F AJF Migot-Nabias Florence F Azouzi Slim S Guerrera Ida Chiara IC Merckx Anaïs A

Frontiers in cellular and infection microbiology 20210324

The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the erythrocyte surface that interact with key human proteins in the Ankyrin R and 4.1R complexes. Oxidative stress generated by HbS, as well as by parasite invasion, disrupts the kinase/phos ...[more]

PMID: 33842387

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Dataset Information

Sickle cell trait modulates the proteome and phosphoproteome of Plasmodium falciparum-infected erythrocyte

Publications

Sickle Cell Trait Modulates the Proteome and Phosphoproteome of <i>Plasmodium falciparum</i>-Infected Erythrocytes.

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