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Phosphorylation dynamics in neuronal cells

ABSTRACT: We performed global phosphorylation quantitative proteomics analysis of neuronal HT-22 cells in response to iron deficiency. Our data applied SILAC-based quantitative analysis with replicates and studied the phosphorylation dynamics under acute, chronic iron deficiency as well as acute hypoxia.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Yue Chen

LAB HEAD: Yue Chen

PROVIDER: PXD023294 | Pride | 2021-09-09

REPOSITORIES: Pride

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Iron Deficiency Reprograms Phosphorylation Signaling and Reduces O-GlcNAc Pathways in Neuronal Cells.

Erber Luke N LN Luo Ang A Gong Yao Y Beeson Montana M Tu Maolin M Tran Phu P Chen Yue Y

Nutrients 20210108 1

Micronutrient sensing is critical for cellular growth and differentiation. Deficiencies in essential nutrients such as iron strongly affect neuronal cell development and may lead to defects in neuronal function that cannot be remedied by subsequent iron supplementation. To understand the adaptive intracellular responses to iron deficiency in neuronal cells, we developed and utilized a Stable Isotopic Labeling of Amino acids in Cell culture (SILAC)-based quantitative phosphoproteomics workflow. O ...[more]

PMID: 33430126

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