Yeast response to protein misfolding induced by azetidine-2-carboxylic acid (AZC)

ABSTRACT: To identify proteins that interact with the yeast Tsa1 peroxiredoxin, we utilized an N-terminal Myc-tagged version of Tsa1 with a mutation in its resolving cysteineresidue (Tsa1-C171S) to trap and detect redox-dependent interactions. Yeast cells were left untreated, or treated with AZC or hydrogen peroxide prior to immunoprecipitation and mass spectrometry used to identify co-immunoprecipitating proteins.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Chris Grant  

LAB HEAD: Chris Grant

PROVIDER: PXD023668 | Pride | 2021-09-10

REPOSITORIES: Pride