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U2OS SLX4IP Knockout Telomere PICh

ABSTRACT: This experiment sought to characterize the perturbations to the telomere proteome brought about by modulation of SLX4IP expression, as we have previously found SLX4IP to act as a key regulator of telomere maintenance.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Nathaniel Robinson

LAB HEAD: William Schiemann

PROVIDER: PXD024141 | Pride | 2022-02-16

REPOSITORIES: Pride

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SLX4IP promotes RAP1 SUMOylation by PIAS1 to coordinate telomere maintenance through NF-κB and Notch signaling.

Robinson Nathaniel J NJ Miyagi Masaru M Scarborough Jessica A JA Scott Jacob G JG Taylor Derek J DJ Schiemann William P WP

Science signaling 20210629 689

The maintenance of telomere length supports repetitive cell division and therefore plays a central role in cancer development and progression. Telomeres are extended by either the enzyme telomerase or the alternative lengthening of telomeres (ALT) pathway. Here, we found that the telomere-associated protein SLX4IP dictates telomere proteome composition by recruiting and activating the E3 SUMO ligase PIAS1 to the SLX4 complex. PIAS1 SUMOylated the telomere-binding protein RAP1, which disrupted it ...[more]

PMID: 34187905

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