Rat myocardial left ventricular tissue proteins LC-MSMS

ABSTRACT: Rat left ventricular tissue LC-MSMS.Rat left ventricular tissue proteins from different groups were extracted and quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific, USA). The proteins were then labeled with tandem mass tags (TMT). TMT6/10 (Pierce, USA) with different reporter ions (126-131 Da) were applied as isobaric tags for relative quantification. Then the labeled peptide aliquots were combined for fractionation and further LC-MS analysis. The LC–MS/MS analysis was carried out in Capitbalbio Technology by using Q Exactive mass spectrometer (Thermo Fisher Scientific, USA). Twenty most intense precursor ions from a survey scan were selected for MS/MS detected in Orbitrap analyser. All the tandem mass spectra were produced by higher-energy collision dissociation (HCD) method. The MS/MS data was collected and searched against the Oryctolagus cuniculus database using the Sequest algorithms. A protein with fold change≥1.5, and the presence of least 2 unique peptides with a P value <0.05, was considered to be differentially expressed protein (DEP). Finally, DEPs were analyzed by reference to three key databases: Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome and the Clusters of Orthologous Groups (COG).

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart

SUBMITTER: Xiaohong Wei  

LAB HEAD: Jingyan Han

PROVIDER: PXD024641 | Pride | 2021-07-26

REPOSITORIES: Pride