Proteomics

Dataset Information

0

Within-host adaptive phenotypic responses of bovine Staphylococcus aureus


ABSTRACT: The Gram-positive bacterium S. aureus is one of the most common causes of contagious bovine mastitis. A predominant S. aureus clone persists within its host over a long period. In the present study, we compared the surface proteome of two isolates, collected from a naturally infected dairy cow with chronic, subclinical mastitis: an initial isolate (IN) and a host-adapted strain (HA). The isolates were grown under iron- and oxygen-limiting conditions in RPMI 1640 medium. Bacterial cells were isolated at the early stationary phase and used for preparation of surface-associated proteins by applying a surface-shaving and a surface-biotinylation approach. Proteins were identified by gelfree (surface-shaving approach) and GeLC-MS/MS (biotinylation approach) analyses.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Martin Kucklick  

LAB HEAD: Susanne Engelmann

PROVIDER: PXD025013 | Pride | 2021-08-14

REPOSITORIES: Pride

Similar Datasets

2021-03-12 | PXD013791 | Pride
2020-08-05 | PXD013951 | Pride
2019-05-24 | PXD009082 | Pride
2021-02-18 | PXD021684 | Pride
2021-05-26 | PXD017932 | Pride
2011-09-29 | E-GEOD-27063 | biostudies-arrayexpress
2012-01-12 | E-GEOD-34441 | biostudies-arrayexpress
2021-09-09 | PXD023445 | Pride
2024-01-26 | PXD044519 | Pride
2011-07-18 | E-GEOD-30757 | biostudies-arrayexpress