Proteomics

Dataset Information

0

Integrative proteogenomics for differential expression and splicing variation in a DM1 mouse model

ABSTRACT: Here, we performed a large-scale coordinated transcriptomic and proteomic analysis to characterize a DM1 mouse model (HSALR) in comparison to wild-type. Our integrative proteogenomics approach comprised gene- and splicing-level assessments for mRNA and protein. It recapitulated many known instances of aberrant mRNA splicing in DM1 and identified new ones. It enabled the design and targeting of splicing-specific peptides and confirmed the translation of known instances of aberrantly spliced disease-related genes (e.g. Atp2a1, Bin1, Ryr1), complemented by novel findings (e.g. Ywhae, Flnc, Svil). Comparative analysis of large-scale mRNA and protein expression data showed remarkable agreement of differential patterns between disease and wild-type on both the gene and especially the splicing level.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle Tissue, Muscle

DISEASE(S): Myotonic Dystrophy Type 1

SUBMITTER: Elizaveta Solovyeva  

LAB HEAD: Sebastian Hoersch

PROVIDER: PXD025589 | Pride | 2024-01-26

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
PRM_long_column_reruns_results.csv Csv
PRM_long_column_results.csv Csv
PRM_short_column_results.csv Csv
PXP0771_Sam10_2D_ES_QE3_2019_1065.raw Raw
PXP0771_Sam1_2D_ES_QE3_2019_1064.raw Raw
Items per page:
1 - 5 of 54
altmetric image

Publications

Dysregulated mRNA splicing is involved in the pathogenesis of many diseases including cancer, neurodegenerative diseases, and muscular dystrophies such as myotonic dystrophy type 1 (DM1). Comprehensive assessment of dysregulated splicing on the transcriptome and proteome level has been methodologically challenging, and thus investigations have often been targeting only few genes. Here, we performed a large-scale coordinated transcriptomic and proteomic analysis to characterize a DM1 mouse model  ...[more]

Similar Datasets

Proteomics Proteomics analysis of monocyte-derived hepatocyte-like cells identifies integrin beta 3 as a specific biomarker for drug-induced liver injury by diclofenac
2018-07-23 | PXD008918 | Pride
Proteomics A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis
2017-10-18 | PXD003281 | Pride
Proteomics Osteosarcoma cells-derived secretome induce pre-metastatic changes in the lung microenvironment that support lung metastasis formation
2024-01-26 | PXD040814 | Pride
Proteomics Operation of a TCA cycle subnetwork in the mammalian nucleus
2022-10-13 | PXD022608 | Pride
Proteomics Preparation of tirapazamine-resistant hepatoma cell line
2023-05-10 | PXD039876 | Pride
Proteomics Identification of interaction partners of the Toxoplasma gondii Cysteine Repeat Modular Proteins TGGT1_292020 and TGGT1_261080
2022-12-13 | PXD031649 | Pride
Transcriptomics Exon array of heart samples of myotonic dystrophic patients
2016-01-21 | E-GEOD-67067 | biostudies-arrayexpress
Proteomics Identification of myelin basic protein interactome using TurboID proximity labeling proteomics
2023-01-25 | PXD039467 | Pride
Proteomics A standardized and reproducible workflow for membrane glass slides in routine histology and spatial proteomics
2023-10-24 | PXD040281 | Pride
Transcriptomics Alternative splicing and gene expression in a mouse model of myotonic dystrophy
2010-01-23 | E-GEOD-17986 | biostudies-arrayexpress